Depending on the cell type and the nature or magnitude of the environmental stress, cells react with stress reaction that actually increases their resistance.
• alteration of the protein profile,
• cell cycle control,
• redox regulation,
• DNA damage sensing and repair,
• lipid and energy metabolism
Several researches have proven that stress treatment by high hydrostatic pressure has improved:
• in vitro maturation of oocytes as observed in the higher cell number of the developed blastocysts,
• blastocyst rates and cell number after enucleation, SCNT and IVC,
• differentiation rate after vitrification of stem cells,
• life span and fertility of boar semen,
• quality of ovine embryos,
• oocyte, semen and embryo survival after cryopreservation.
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Hydrostatic pressure stress treatment has improved post-thawing survival of
• porcine, mouse and human oocytes,
• mouse and bovine embryos,
• bull and boar semen,embryonic stem cells,
• cloned porcine blastocyst, ovine blastocysts.
Hydrostatic pressure, unlike all other environmental stresses
• acts immediately and uniformly at each point of the sample,
• is not related to penetration problems or gradient effects,
• can be applied with the highest precision, consistency, and reliability.
Please, study the related publications in the Research menu.
Hydrostatic pressure is transferred instantly to the cells. The change of the pressure parameters during the treatment also takes effect immediately: as e.g. decompression to atmospheric pressure (0.1 MPa) ceases the direct pressure effect in the cells right at the moment, when the set value reaches 0.1 MPa.
Experiments revealed that unlike, for example temperature, where even a 2–3°C increase in the physiological temperature might reduce viability, hydrostatic pressure acts with a wide safety margin, E.g., treatment of bovine blastocysts was effective at 40 MPa, but even an 80 MPa/30 min treatment caused no loss in the continued in vitro development and hatching rate.
